FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15079606
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182500.12102438220202837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG39050.017.3968581
GTATTAG28950.016.5573881
GTGCTAG37450.016.390561
GTTCTAG45800.015.5105561
GTCCTAA33750.015.3265051
GTACTAG17650.015.0442891
GTACTAA26550.014.8069611
GTATTAA41250.014.7970431
GTCTTAG34100.014.7646821
GTATAGG26000.014.5896531
CGATCGT5650.014.028913538
GTTCTAA47850.013.5488221
GTCTTAA40300.013.4344611
GGTCTAA29650.013.3751541
GTATATA50350.013.2185391
GTGTTAG36800.013.1191541
CTATACT31450.013.0375534
GTGCTAA35000.013.0056341
CTAGACC29250.012.9579584
GCCTTAG35750.012.8292491
Produced by FastQC (version 0.11.5)