FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10624143
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12850.016.3251131
GTCCTAG27050.015.3832341
GTATTAG22200.015.3359881
GTTCTAG32300.015.2252021
GTATTAA33600.014.9431821
GCTCTAG31700.014.2115531
GTATAGG18600.013.6819591
GTACTAA22100.013.3824521
GTGCTAG28450.013.0548241
GTCTTAG23450.013.0519891
GTACAGA49400.012.9483821
GTGCTAA27600.012.709271
GGTCTAG20900.012.6699221
GTACAGG45750.012.4028741
ATTAGAC22800.012.369133
GTCTAGG25250.012.2577471
GTCCTAT22950.012.1375731
GGTCTAA21200.012.00398351
GTTCTAA33000.011.8801091
GTCCTAA22900.011.8637271
Produced by FastQC (version 0.11.5)