FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10755529
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21750.017.5552751
GTCCTAG29050.017.525061
GTGCTAG25350.015.6051
GTACTAG11600.014.5305481
GTCCTAA23800.013.7306331
GTCTTAG22900.013.5191981
GGTCTAG21100.013.3682741
GTTCTAG30000.013.3008881
GTATTAA33100.013.1983511
GTACTAA20450.013.1203441
GCCCTAG27300.013.1043241
GTACTAT21500.012.9595671
GTATATG32400.012.74031451
GGTCTAA18650.012.5422081
GTTCTAA31300.012.5286551
GTATAGG18750.012.4753161
TCTATAC23450.012.1760223
GTATATA42650.012.0980721
GTACAGG44800.012.0549551
GCTCTAG31000.011.9841171
Produced by FastQC (version 0.11.5)