FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10755529
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23050.017.8141081
GTACTAA22400.015.71231651
GTCCTAG25950.015.6903531
GTACTAG14300.014.719121
GGTCTAG19900.014.5651271
GTATTAA35550.014.5592741
GTGCTAG25500.014.3434531
GTCCTAA23300.013.9206661
GTATATA41350.013.8522611
GTATATG32950.013.4042531
GTGCTAA25950.013.2969091
GTTCTAG32850.013.2349781
GTGCGTA5400.012.7798071
GTACATA29650.012.5686061
GTACAGG45350.012.4782781
CTAGACT24000.012.4774534
GCCCTAG26300.012.4639571
TATACTG30150.012.4377285
GTCCTAT24450.012.419151
TCTATAC24150.012.4091333
Produced by FastQC (version 0.11.5)