FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454743
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG15250.023.2995821
GTCCTAA14350.021.7289351
CGTCTTG21900.020.525393145
GTACTAA13950.019.752921
GTATTAG16550.019.7167971
CGTCTTA12900.019.670809145
GCCCTAG16300.019.1294611
GCGCTAA4404.8677975E-818.1285021
GTATTAA25350.017.1630781
CTTAACG6404.5474735E-1116.9943263
GTACTAG8550.016.962341
GTATATA24900.016.8908121
GGTCTAG12450.016.8908121
TCTAGAC13800.016.813713
GTTCTAG17850.016.6558781
GTATAGG13100.016.606261
CTAGACT13300.016.3550744
GTCCTAT15150.016.2737711
GTCTAGG15350.016.0617331
CCCTAGG18350.015.8056252
Produced by FastQC (version 0.11.5)