FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454743
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT7850.025.847063145
GTCCTAG14750.024.1220951
GTATTAG16800.022.4752751
GACGATA17000.021.312967145
GTACTAG8650.020.1468031
GTCCTAA15900.020.0940061
CCCTTAG16500.019.3633141
GTATTAA24850.018.4087831
CTATACT16250.018.2885844
CGCCGTT14450.017.551855145
GTATAGA17000.017.512411
GCTCTAG19100.017.487811
GTACTAA14600.017.4070871
GTGCTAG15900.017.3539141
GTTCTAA21800.017.3203961
TTATACC31800.017.318462145
ATAGTAC14150.016.910193
TCTATAC16450.016.7497653
GTACTAT14500.016.5255891
GTACATA18600.016.3963551
Produced by FastQC (version 0.11.5)