FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4444307
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG23300.021.78249145
GTTCTAG18200.020.3192731
GTCCTAG15200.020.0361881
CGTCTTA14700.019.729134145
GTACTAT12600.019.5667081
GTACTAA13050.019.447641
GTATTAA24850.018.96691
GTACTAG8850.018.8449061
ACTATAC13500.017.7217453
GTATAGA16450.017.1912751
GTACTGA22850.017.1362951
ATAGTAC13150.017.0907963
GTCTAGA14500.017.0027941
GGTCTAG11950.016.990241
GTGCTAG14550.016.9443651
CTATACT15050.016.8600124
GTCCTAC16400.016.801541
TCTAGAC13150.016.5394783
GTATATG21800.016.298551
TCTATAC16050.016.2612463
Produced by FastQC (version 0.11.5)