FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4253308
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG16850.022.8088321
TAACGCG2603.2077587E-622.308075
GTACTAA13600.021.3279021
GTACTAG8250.021.0952341
GCTCTAG18650.019.4409851
GTTCTAG17350.019.2258431
GGTCTAA13250.019.1548711
GCCCTAG14200.018.384051
CTATACT15200.018.1253054
CGTCTTG25300.017.7671145
GTATATA22400.017.4812621
CGTCTTA16800.017.262197145
TAGACAG22150.017.0206055
GTCCTAG13700.016.937781
GTCTTAG16500.016.7003941
GTCTATA13150.016.5433161
GTATAGA16350.016.4100931
TCTAGGC17550.016.1113853
ATAGTAC11800.015.9748463
GTACATA17750.015.9328441
Produced by FastQC (version 0.11.5)