FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4253308
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT9300.028.062464145
GTTCTAG19500.021.94411
GTACTAG8500.021.3315011
CCGTATA9850.020.607632145
GGTCTAG12300.020.0481451
GCCCTAG13400.020.0261421
GTACTAA13100.019.9311141
TATACGG5555.456968E-1219.5906285
GTCTTAG15500.018.7166731
GACGATA19250.018.076601145
TCTAGAC14050.018.0562063
GTCCTAG16000.017.6784841
CTAAGCG6354.1836756E-1117.1223164
GTGCTAG15400.016.954391
GTATTAG15000.016.9229931
CTAGACT12500.016.8163994
GTGCTAA17800.016.7056831
GTACAGG31400.016.6304191
TATACCG4451.0922286E-616.2888375
CAGTACT24050.016.27514
Produced by FastQC (version 0.11.5)