FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4316251
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15600.024.1718921
GTACTAG7800.021.3828281
CGTCTTA18250.019.862936145
GTACTAA14400.019.6396621
CTAGACT12300.019.4511434
GCTCTAG17550.019.4201531
GTGTATG24650.019.4159621
GGTCTAA12800.019.2619761
TGCCGTG28800.019.13187145
GGTCTAG12250.018.9428711
GTATAGT12100.017.9790941
GTACTAT13350.017.9252221
GTTCTAG18400.017.7348121
GTTCTAA21300.017.7033581
GTCCTAA15650.017.6076411
GTGCTAG15300.017.0625131
GGGTTAG15300.017.0625131
CGTCTTG25950.017.042322145
GTCTTAG17100.016.9627321
TATACTG21650.016.4087125
Produced by FastQC (version 0.11.5)