FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4316251
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG8400.024.1752551
GTCCTAG16050.023.9493181
GTACTAA13750.023.7357041
CCGTATA10350.023.112904145
GTGCTAG14900.022.8772541
ACGCGTT9850.021.342371145
GTCCTAA14000.021.2396891
GTATTAG15400.021.1925951
CTAGACT14200.020.9320454
GTCTTAG15300.020.3830591
GTTCTAA20700.019.9708631
GCTATAC11500.019.542723
GCTCTAG18900.018.8029751
CGCCGTT15450.018.767757145
GTCCTAT14700.018.7481561
GTGCTAT17700.018.4387531
TCTGTAC29750.018.0329133
TAGATCG8700.017.4977145
CTGTACT28900.017.3088174
CCTAGAC13850.017.2736973
Produced by FastQC (version 0.11.5)