FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15887115
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG26500.018.6575431
GTACTAA26300.017.4661331
GTACTAG17250.016.4655841
GTCCTAG36400.016.2804551
GTATAGG24600.015.2521081
GTTCTAA42500.015.0988331
GTTCTAG43100.014.9699981
GTATTAA40800.014.9544451
GCTCTAG43950.014.9198321
GTCTTAG35800.014.7902371
GTGCTAG37800.014.0076861
CTATACT34850.013.9615134
GGTCTAA26750.013.8951521
GTGCTAA36350.013.8911841
TCTATAC28850.013.7147253
GTCTAGA32700.013.5115141
GTCCTAA29950.013.3471711
GTATATA40900.013.2889181
GTGTTAG34650.013.2571231
GTATAAG31400.013.0658431
Produced by FastQC (version 0.11.5)