FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15855819
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36650.017.859251
GTATTAG27950.016.2801021
GTTCTAG39650.016.0665911
GTCTTAG33200.015.7088271
GTACTAG18350.015.450571
GTATTAA42200.014.9298551
GGTCTAA26250.013.8675451
GTGCTAG34850.013.8602761
TATACTG44250.013.8432975
CTATACT34150.013.8372434
TCTAGAC31550.013.5351443
GCGATCG8800.013.52273237
GTACAGG72450.013.382491
GTACATA37500.013.3475121
GTACTAA26250.013.3341781
CGATCGT8050.013.04344138
GTATATA44300.012.878921
GTCCTAA34050.012.8495661
GTATATG41300.012.79742051
GTCTTAA36800.012.6502581
Produced by FastQC (version 0.11.5)