FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15855819
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36200.017.3371
GTATTAG24500.017.3160481
GTACTAA27550.016.671671
GTACTAG19000.015.68536951
GTTCTAA43650.015.4222041
GTCCTAA32600.015.3797021
GTTCTAG41450.014.7181831
GCTCTAG46300.014.5395071
GTATTAA44350.014.2301121
GTGCTAG35650.014.0639071
GGTCTAG26950.013.6602921
GTATAGG24400.013.5072681
GTATTAT42450.013.4629241
CTATACT33700.013.2933684
GCTCTAA40700.013.2664811
TCTAGGC49150.013.249033
GTGCTAA37050.013.2485781
GTATATA44050.013.2127021
GTACAGG67550.013.131811
GTCTTAG32950.012.8753611
Produced by FastQC (version 0.11.5)