FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15807289
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17900.020.5439131
GTATTAG28050.018.8534551
GTCTTAG31350.016.1985951
GTCCTAG38800.016.1572781
GTTCTAG43150.015.4212541
GTATAGG23450.014.7856091
GTACTAA25850.014.4967321
GCTCTAG44100.014.3743051
CTATACT34600.014.2620984
GTATTAA41900.014.2095981
GTCCTAA31850.013.8550471
GTGCTAG37850.013.6018611
GTATATA45300.013.4523421
GGTCTAA26650.013.4044761
GCCCTAG37600.013.3197191
GTGCTAA35300.013.2946521
GTTCTAA41900.013.2901531
GTCCTAT34150.013.2295761
GTACAGG69500.013.1523281
GTCTTAA42000.013.008351
Produced by FastQC (version 0.11.5)