FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15789127
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17650.018.651861
GTATTAG25500.018.2662931
GTTCTAG45800.016.3639161
GTATAGG22000.015.9190181
GTCCTAG36200.014.6085591
TCTATAC31150.014.6072463
GTCCTAA30900.014.5074291
GTTCTAA43050.014.236521
CGATCGT7150.014.1958938
GTACATA37000.013.9140821
GGTCTAA26300.013.8489411
GGTCTAG29800.013.7501861
GTACTAA28700.013.667061
GTCTTAG32300.013.5533441
TCTGACG128450.013.51451170
GTCCTAT31900.013.5037181
GTGCTAA34800.013.3847841
TAGTGCG9600.013.12437357
GCTCTAA41450.013.0962251
CTATACT32450.013.0511744
Produced by FastQC (version 0.11.5)