FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12677779
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG31650.016.1465991
GTATTAG21350.016.0668281
GTATATA33550.015.8581671
GTACTAG14650.015.5302211
GTCTTAG26500.015.321951
GTCCTAA28550.015.2025851
GTCTATA21400.014.7207741
TCTAGAC27350.014.7180743
GTGCTAG28400.014.5433891
GTACTAA21100.014.4324051
GTATTAA31900.013.9352561
GGTCTAA22200.013.8749561
GGTCTAG23100.013.6374281
CTAGACT27300.013.5912864
GTATATG34050.013.4665421
GTGTAGG45300.013.4447571
GCTCTAG36200.013.2468891
GTATAGG19950.013.1589211
GTACTAT22900.013.145131
GTCTAGG29600.013.1260231
Produced by FastQC (version 0.11.5)