FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12538886
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12900.018.188661
GTACTAA22200.017.0367361
GTCCTAG31850.016.3829631
GCTCTAG36600.016.26611
GTATTAA31550.015.983751
GTATTAG21350.015.7466781
GTGCTAG27900.015.4389061
GGTCTAG24250.015.16328051
GTCTTAG23900.014.9457561
GTCTTAA28700.014.8865331
GTTCTAG34350.014.5789051
GTCCTAA26450.014.5640651
GTATAGG19600.014.1151881
TCTAGAC26450.014.0253953
GTCTTAT29950.013.7975131
TCTGTAC54200.013.6244243
GTGCTAA27900.013.4305931
GTGTTAG28250.013.3881591
GTCCTAT26200.013.3663961
GTATATA36300.013.2168991
Produced by FastQC (version 0.11.5)