FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12540755
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14900.018.802351
GTCCTAG29450.018.3123381
GTATTAA33800.016.1627881
GTATTAG22600.016.1151081
GTTCTAG32100.015.9278151
GTCTTAG25950.015.7890821
GTGCTAG29300.015.6571251
GTCCTAA24500.015.5800481
GGTCTAA22000.015.281181
TCTATAC24650.015.0511143
GTACTAA22750.014.7774061
CTAGACT25050.014.5312144
GCCCTAG31550.014.0965461
GTATAAG21500.013.8448681
GTATATA35950.013.7349971
GTTCTAA34750.013.7054241
CTATACT27200.013.6399644
GTATATG31150.013.3781881
GTGTTAG29200.013.3121581
CTAAGCG8200.013.23195654
Produced by FastQC (version 0.11.5)