FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8491930
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG62150.029.865458145
GTATTAG28250.022.3305441
TCTCGTT44300.021.767988145
TGCCGTG68050.021.415997145
GTACTAG16400.021.2224671
GTCCTAG34000.021.1132321
GTGCTAG33300.018.2908341
GTTCTAG37700.018.0794431
GTCCTAA29500.016.959991
GTATAGG24000.016.9190241
GTTCTAA40350.016.5326611
GTGCTAA32200.016.4386251
GCTCTAG39400.016.3791851
GTACAGA63400.016.3548011
GTACTAA25300.016.0496651
GGGGGGG3874100.015.99981145
GTATAAG29700.015.8692121
CTATACT31100.015.851874
GTATTAA43600.015.7992181
GTATAGA30850.015.7477351
Produced by FastQC (version 0.11.5)