FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8329064
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG76300.023.84998145
GTCCTAG34550.023.0876621
GTACTAA25100.021.66821
TGCCGTG86150.020.95477145
TCTCGTT53850.019.656492145
GTACTAG17500.019.0614131
GTATTAG27050.018.7657571
GTCCTAA27900.018.713871
GTGCTAG33700.017.8600851
GTACAGG62800.017.7826461
GTATTAA44600.017.722581
GTTCTAG39700.017.5354141
GTATAGA30150.016.8362751
GGGGGGG3696200.016.235151145
GTATAAG28750.016.1427461
GTATACG6801.1641532E-1015.9962311
GCTCTAG38550.015.9893151
TCTAGAC28200.015.9395553
GTGTAGG43900.015.8577671
GTTCTAA43000.015.6837461
Produced by FastQC (version 0.11.5)