FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10362801
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT15750.029.446495145
CCGTATA16000.028.533482145
GTCCTAG45000.026.1449011
TACGCGA3702.582965E-1023.5341632
GGTCTAG34350.020.0854421
GTCCTAA38300.019.9101751
GTACTAA33850.019.738481
GTGCTAG43050.019.7377321
GTTCTAG45850.019.4827521
GTATTAA53700.019.4747871
GTCTTAG37400.019.4183771
GTATTAG33850.018.4511871
GTATAGG27500.017.4299351
TCTAGAC41350.017.363183
CTGCCGC164750.017.242289145
GTACAGA75800.016.8627341
GTACTAG19450.016.8026371
GTATAAG35350.016.6410271
GTTCTAA50200.016.6371381
GTCTAAG39100.016.5309491
Produced by FastQC (version 0.11.5)