FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10429954
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT16000.031.699476145
CCGTATA16600.027.498344145
GTATTAG37050.020.1803911
GTGCTAG40750.019.0606061
GTTCTAG47600.018.9101621
GTCCTAG44050.018.7862241
GCTCTAG50700.018.756161
GTATTAA50050.018.7096711
GTACTAG20600.018.6762311
GTTCTAA47050.018.6683651
GTACTAA30850.018.5888481
TCTATAC38050.017.911183
GGTCTAA33100.017.3252561
GTACAGG76300.017.2200581
GTCTTAA48400.017.0977941
GTCCTAT39250.017.0148741
TTATACC70000.016.871885145
GTCCTAA36600.016.8584791
CTAGACT37700.016.7244534
GTATAGG26950.016.6998861
Produced by FastQC (version 0.11.5)