FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10260012
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT18400.029.548145
CCGTATA20850.024.337538145
GTCTTAG37900.022.7731591
GTACTAA31650.021.3120271
GTCCTAG42400.020.5272541
GTACTAG22000.019.451131
GTATTAA50700.018.8834551
GTATTAG34950.018.6771581
GTGCTAG42450.018.2819121
GTCCTAA38650.018.202781
GTGCTAA39550.017.9719451
GCCGTAA16700.017.797215145
CTATACT40050.017.193384
GGTCTAA32000.016.9991321
GTTCTAG47650.016.591251
GTTCTAA50500.015.7985341
TTATACC96950.015.253413145
GTCTAGG39050.015.2302951
CTACACT46300.015.1855654
GCCGACC41550.015.17864145
Produced by FastQC (version 0.11.5)