FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10335694
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG50300.020.17884145
GTCCTAG41200.019.008361
GTTCTAG47500.018.9298061
GTACTAG20050.018.8064611
GTATTAG37200.018.1283441
CTATACT40000.017.9436724
GTCTTAG37500.017.7899481
GTATATA59350.017.7159881
GTACTAA31900.017.5032251
GTCCTAA37350.017.47311
GTGCTAA38150.017.2967661
GTATTAA50100.017.2237341
GTGCTAG40650.016.9465451
GTATAGG25450.016.8105641
GGGGGGG4443300.016.804499145
CGTCTTA31200.016.730698145
GTACTAT31950.016.3410421
GTTCTAA48000.016.3155081
GGTCTAG33750.015.6843741
ATAGTAC29850.015.5443223
Produced by FastQC (version 0.11.5)