FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9439229
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA71150.032.47471145
GGGGGGG602350.025.099329145
GTTCTAG41050.015.5425641
GCGTATG17451.9003868E-514.712357145
GTGCTAG37850.014.6768661
GCGCTAG6751.8189894E-1214.6671781
GTCTTAG34500.014.5077521
GTATTAG32500.014.3851171
GTCCTAA31050.013.9940461
GTATTAA43750.013.5776171
GTCCTAG36800.013.30209351
GCGCCTA13100.00937735513.065172145
GTACTAG16200.012.9016851
GTATAGG25000.012.7604451
ATGCCGT42550.012.737647145
GTACTAT27550.012.5775711
GCCCTAG35150.012.5182181
GGTCTAG30800.012.5004361
GTTCTAA42400.012.4532641
GTATATA55100.012.3779271
Produced by FastQC (version 0.11.5)