FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9557159
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA66600.036.80267145
GGGGGGG632050.024.225775145
TATGCCG31650.018.221783145
ATGCCGT39500.017.52059145
TCGTATG31850.015.391257145
TATCTCG22900.015.110552145
GTTCTAG41750.015.0135551
GTATTAG32400.014.9338851
CTCGTAT30950.014.9071245145
GTACTAG18500.014.8604991
GTCCTAA30450.014.8068171
GTATAGG24450.014.6173841
GTCCTAG36100.014.4694351
GTCTTAG35050.013.6479181
GTGTTAG42000.013.6150471
CTAGACG9050.013.36624054
TGCCGTA15250.001555062413.236183145
GCGTATG15301.4375801E-613.192927145
GTGCATA33700.013.05254651
GGCGTAT11102.942294E-412.989176145
Produced by FastQC (version 0.11.5)