FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9557159
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA26400.023.36998145
CTGTCTA69150.023.116508145
CCTGTCG31550.019.555227145
TGACGCG14453.7277368E-617.466837145
CGCCGTT13053.0389867E-517.19171145
ACGGTGT66850.015.521722145
GACGAAT74650.015.402586145
CGAATTT19153.3043834E-614.644374145
GTACTAA26550.014.552991
GCTGTCG38750.014.474311145
GTGCTAG37250.014.0771921
GTTCTAG44750.014.0614631
GTATAGG23050.013.88915351
CCGACGA74800.013.872021145
GTCTTAG35100.013.6814111
GTTCTAA42150.013.4883361
GCCGTAT27100.013.452829145
CGTATCA25650.013.119988145
GTACTAG18350.012.9345431
GCTCTAG42850.012.8815431
Produced by FastQC (version 0.11.5)