FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304800
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG38150.034.954952145
CCGTATA10651.2732926E-1130.051414145
CTGTCTA73300.023.28677145
TCGCCGT42450.018.848501145
AAGGGGG76750.017.027504145
GTCTCGT33650.016.644377145
GTACTAA26750.016.3467621
GCCGTAA13255.3235963E-516.103024145
GCCGTAT35500.015.776994145
GACGAAT110600.014.950987145
GTCCTAG35200.014.9385821
TAGATCG10851.4296109E-514.748736145
GTTCTAG40300.014.5589231
TCGCGGT16602.2377939E-514.459982145
CGCCGTA34050.014.099011145
ACGGTGT89000.014.084491145
TCTATAC33400.014.0808263
GTATTAG31450.013.9038441
TCGGTGG83100.013.800688145
GCTCTAG42150.013.78861
Produced by FastQC (version 0.11.5)