FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10682386
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12650.017.952221
GTCTTAG23750.015.2121441
GTTCTAG29800.014.7794561
GTATTAG19500.014.6456361
GTCCTAA20150.014.3439581
GTACTAA19200.014.33684351
GTATTAA29750.013.9946861
GTGCTAG25000.013.76337051
GTACAGG46700.013.7044261
GTATTAT28250.013.6415691
GCTCTAG30900.013.3624941
GTCCTAG26600.013.0648531
GTATATA32250.013.01652051
GTACATA29050.012.7921151
CTACTAG13450.012.7912361
GGTCTAG19950.012.7630241
GTCTTAA27900.012.7027511
CGATCGT3852.3608663E-812.516314538
GTATAGG17350.012.4941251
TACTAGG15700.012.4923482
Produced by FastQC (version 0.11.5)