FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344828
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10900.017.3640231
GTATTAG18450.016.973021
GTCCTAG22700.015.6144081
GTATATA32700.014.3121631
GTATTAA28600.014.1980961
GTACTAA19700.014.1492331
GTTCTAG30000.013.7649351
ACTATAC17000.013.360473
TCTAGAC24250.013.3396013
GTATTAT26850.013.3291721
GTACATA27550.013.2403181
GCTCTAG30950.013.00886251
GTGCTAG24150.012.8244721
GTCCTAC24750.012.65261651
GTATATG28100.012.4913091
CTATACT22700.012.1282824
GTACAGA50550.012.1174991
GTACAGG49500.012.09645751
GTATAAA38700.012.0932241
TATACTG26500.012.0774825
Produced by FastQC (version 0.11.5)