FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344828
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139110.13447299462108023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA2002.9102011E-818.9618762
GTTCTAG28600.017.3703921
GTACTAG11250.016.8664361
GTCCTAG27750.016.7835541
GTATTAA26900.015.64662551
GTGCTAG24900.014.6865461
GTCCTAA22900.014.6133371
GTCTTAG23100.014.48681451
GTATATG27500.013.9252641
GTATATA35100.013.7604951
GGTCTAA18200.013.6481651
GTCTTAA23650.013.4205351
GTACTAT18850.013.3605591
GTTCTAA29300.013.1875321
GTATACG4204.1472958E-1013.1426771
CTAGACC20350.013.0262274
GTATAGG15400.012.9933291
GTATTAG20250.012.947971
GTCCTAT22750.012.8899331
GTATAAG20100.012.8729581
Produced by FastQC (version 0.11.5)