FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396897
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19050.019.0037861
GTATTAG31150.016.8240811
GTCCTAG38550.015.9199391
TATACTG46100.015.2572075
GTATATA55950.014.8512411
GTACTAA29650.014.5355381
GTATTAA45400.014.4292431
GTATAGA33700.014.118711
GTCTTAG36400.014.0186491
GCCCTAG38200.013.8996281
GTCCTAA31900.013.8345521
GTGCTAG37450.013.7176681
GTATAGG26150.013.7122091
GCTCTAG44400.013.6670721
GCGATCG6800.013.18390637
GGTCTAG29150.012.89241
CGATCGT7000.012.80797238
GTGCTAA36350.012.804871
GTTCTAG42350.012.78181
GTTCTAA47250.012.7697391
Produced by FastQC (version 0.11.5)