FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15432067
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18700.019.1757951
GTATTAG31500.016.5282941
GTCCTAG36600.016.4861261
GTATTAA47550.015.2275641
GTACTAA28900.014.9133021
GTATAGG22650.014.7660791
GTCTTAG35950.014.4823731
GCCCTAG37800.013.9560111
GCTCTAG44000.013.7918231
GTATATA58450.013.4496811
CTATACT36150.013.3516514
ATAGTAC27450.013.3130863
GTTCTAG42500.012.899411
GCCTTAG37000.012.8599431
GTACTAT28800.012.8101131
GTATAAA60250.012.7617271
GTGCTAG36750.012.7597811
GTGTTAG39800.012.5616471
GTATATG46550.012.5178191
TCTATAC33950.012.4905213
Produced by FastQC (version 0.11.5)