FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14726828
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18000.019.0201871
TAGTGCG8300.017.72316457
GTCCTAG38250.016.6356011
GTTCTAG41750.016.1521451
GTGCTAG35000.015.5125681
GTACTAA29300.015.4616471
GTCTTAG30900.015.4444591
GCGATCG5850.015.33717537
GTATTAG27950.014.723711
GTATTAA43700.014.4817731
TAGTCGG11100.014.2983569
CGCGAGA10500.014.12961717
GCTCTAG43050.013.9774571
GTCCTAA33200.013.7495341
CGATCGT6750.013.29280738
GTGCTAA37300.013.0726121
CTATACT34250.012.897674
TCTATAC31600.012.8923573
TCTAGAC32550.012.8342893
TACTAGA30700.012.7179012
Produced by FastQC (version 0.11.5)