FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14640972
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17850.018.1748281
GTCCTAG36550.017.2800251
GTACGCG5400.015.9781651
GTGCTAG34850.015.5481081
GTATTAG31950.015.0149741
TAGTGCG9050.014.6972757
GTCTTAG35850.014.4405181
GTATATA52750.014.1322711
GTACTAA29100.014.1134971
GTCCTAA30950.013.8274381
TACGCGA4304.1836756E-1113.6445642
CGATCGT5350.013.55010438
GGTCTAG26800.013.521821
GCTCTAG43300.013.4703671
GTTCTAG42250.013.3966991
TAGTCGG11050.013.1184049
TCTATAC29300.013.0747843
GTCTTAA43650.012.9670231
GTACATA39500.012.9313931
GTTCTAA42700.012.7705581
Produced by FastQC (version 0.11.5)