FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14640972
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18000.016.1438671
GTGCTAG36200.015.8635491
GTCCTAG38400.015.4051421
GTATTAG28600.014.877841
GTTCTAG43450.014.8089391
GTCTTAG33500.014.560461
GTATAGG23900.014.4744781
GTTCTAA42350.013.9682981
GTCCTAA32700.013.2240081
CTATACT32000.012.8375294
GTACTAA30750.012.8250931
GCGATCG6750.012.78445237
GTACAGG62400.012.7509941
GGTCTAG28750.012.6343321
GCTCTAG44650.012.6289411
GTCGACG7450.012.58485751
GTCCTAT31900.012.5796361
TATACTG43400.012.564715
GTATTAT48350.012.3064491
TTATACT49100.012.303854
Produced by FastQC (version 0.11.5)