FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14462845
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36700.017.8845121
GTATTAG31500.016.779171
GTGCTAG35750.015.7507471
GTACTAG18350.015.6254161
GTACTAA28100.015.3671481
GTCCTAA29950.015.1099851
GTTCTAG41650.015.0954461
GTCTTAG32800.014.5343231
GTATAGG22650.014.4892191
GTCCTAT29350.014.0064621
GGTCTAG28550.013.914941
GTATTAA43500.013.8975531
GCTCTAG46300.013.8032171
CGATCGT6450.013.37846638
GTTCTAA43500.013.02399351
TATACTG45250.012.966925
GGTCTAA27550.012.915321
GTACTAT27150.012.8511241
GCGATCG6450.012.84314937
TAGACAG58200.012.6317385
Produced by FastQC (version 0.11.5)