FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14254874
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG30150.015.91140651
GTACTAG18100.015.8263461
GTATTAG29350.015.4043831
GTCCTAG38100.015.1276891
GTACTAA29400.014.67384051
GTGCTAG36400.014.4119691
CGAATCG6500.014.3362189
GTTCTAG43650.013.9949111
GGTCTAG29000.013.924161
CGATCGT6950.013.90739338
GCGATCG6950.013.90671237
GTATTAA45700.013.8202531
GTCCTAT30150.013.5075251
GTCTTAG31100.013.4278381
GCCCTAG34850.013.0723091
GGTCTAA25400.012.9083581
GCTCTAG43100.012.7321271
GTATATG42350.012.631631
TATACTG43050.012.5867535
GTATATA53350.012.5501361
Produced by FastQC (version 0.11.5)