FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14254874
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36450.016.7751031
GTACTAG17400.016.0814511
GTATTAG33450.015.6977331
GTCTTAG33300.015.2497431
GTTCTAG40450.015.1162541
GTGCTAG34200.014.6464161
CGATCGT5450.014.56920238
GTCCTAA30650.014.5394741
GTATATA51350.014.0603241
GCTCTAG42550.014.0454611
GTATTAA45100.013.7108991
TCTAGAC33000.013.7042153
GTATAGG22750.013.3625941
GTACATA37750.013.1775611
GTTCTAA44150.012.9104891
GTATAAG29700.012.7945711
GTACTAA28700.012.7589061
GGTCTAG28700.012.6385391
CTATACT31350.012.333074
CTAGACT33100.012.3067884
Produced by FastQC (version 0.11.5)