FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14805896
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30400.017.2109031
GTACTAG18000.016.4459741
GTCCTAG34150.016.0265521
GCGATCG6500.015.35825137
CGATCGT6300.014.75414638
GTGCTAG33650.014.5256941
GTATAGG23100.014.3051661
GTCCTAA31400.014.1414421
GTTCTAG41500.014.1004981
GTATAAG30750.013.8806641
GTATTAA43250.013.848311
GTACTAA27500.013.7687231
GTACAGG67900.013.5862211
GTATATA49900.013.5203891
GTTCTAA47200.013.126961
CTATACT33000.012.5170214
GCTCTAG43600.012.3950091
GGTCTAA26850.012.3072381
GTCTTAG32400.012.1113771
CTAGACC28650.012.0145914
Produced by FastQC (version 0.11.5)