FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14805896
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA28450.017.5930861
GTACTAG18200.017.2594261
GTCCTAG36450.017.1410471
GTATTAG28150.016.7995831
GTGCTAG36950.015.040691
GTTCTAG41350.015.0263421
CGATCGT6050.014.79455538
GGTCTAG27000.014.4467771
CGAATCG7150.014.4388759
GTATTAA43050.014.4329681
GCGATCG5800.014.24395237
CTAAGCG11050.014.0259494
GTATAGG23550.013.7782241
GTCCTAA32100.013.7645261
CTATACT34050.013.756374
GCTCTAG45500.013.7316741
GTCTTAG33100.013.6615381
GTATATA48950.013.5395691
GTGTTAG35950.013.4426671
TATACTG42200.013.2970755
Produced by FastQC (version 0.11.5)