FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14848578
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG37700.017.0760921
GTATTAG30050.016.611611
GTACTAA28600.016.4908391
GTTCTAG42200.015.9078081
GTACTAG17450.015.5854681
TCTATAC30600.014.1754653
GGTCTAG28200.014.0390251
GTCTTAG33400.014.0178271
GTGTTAG35800.013.8473831
GTGCTAG37700.013.7887161
TACGCGA3801.3478711E-913.5892622
GTCCTAA31250.013.4399611
GTATATA52350.013.2180561
TATACTG42250.013.2003455
GTATTAA42650.013.0762791
CTATACT34150.012.8028624
GTATATG43900.012.7823681
GTTCTAA43450.012.5978251
GTACAGG64750.012.5475931
GCCCTAG36500.012.5443161
Produced by FastQC (version 0.11.5)