FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14664682
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14550.020.8207451
GTCCTAG37050.017.2822911
GTATTAA43000.015.2911971
GTACTAA28100.015.1911961
GTATTAG28950.014.7451681
CTATACT33450.013.9965464
GTATATA51100.013.8778731
GTCTTAG32500.013.8760081
GCTCTAG41900.013.8029481
GGTCTAG30950.013.5698731
GTTCTAA43950.013.4724371
TACGCGA3102.924171E-713.3258882
GCCCTAG32650.013.2850721
GTGCTAG36850.013.2656161
TATACTG41400.013.2214575
GTGTTAG33800.013.1386161
GTCCTAA33150.012.8770021
GTATAAG27700.012.800711
CTAGACT32550.012.7971264
GTGCTAA39350.012.5977891
Produced by FastQC (version 0.11.5)