FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13919564
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG33350.017.3815291
GTATTAG25450.015.9981131
GTACTAG16500.014.847351
GTATATA44400.014.6099731
GTCCTAA29100.014.3471921
GTATTAA38600.014.3023441
GTCTTAG31850.014.3000991
GTGCTAG34050.014.2881661
GTTCTAA39350.014.1174311
GCTCTAG40000.013.7155011
GTACTAA24250.013.6594751
TATACTG36050.013.0753135
GTACTAT23850.013.020531
GTTCTAG36950.012.9800051
GTATAGA28350.012.9011161
TCTATAC27500.012.6509183
TCTGTAC57200.012.5256613
GTATAGG21250.012.5027721
GTGCTAA32950.012.4613791
GGTCTAA26200.012.2477461
Produced by FastQC (version 0.11.5)