FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13847814
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG33400.016.684691
GTATTAG26100.015.9475691
GTATTAA36500.015.8331031
GTCCTAG33750.015.6962741
GTACTAG15400.015.6360451
GTATATA42500.014.9738141
GTTCTAG38200.014.6782361
CGAATCG6100.014.662059
GGTCTAG26300.013.9951531
GTCTTAG28850.013.9504971
CTATACT30600.013.7148524
GTACTAA22300.013.5746121
GTTCTAA36650.013.5156871
GGTCTAA24700.013.3697691
GTATAAG27850.013.3397661
GTGTTAG34700.013.0856121
TATACTG38450.013.0620825
GTACTAT25150.012.8569961
GTATAGG19700.012.746951
GTCTTAA33600.012.6949811
Produced by FastQC (version 0.11.5)