FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13847814
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17450.017.402531
GTTCTAA36100.015.8681421
GTCTTAG32500.015.5022481
GTATTAG25200.015.0632021
GTCCTAG33600.014.8920291
GTTCTAG38600.014.3040111
GTGCTAG35700.014.0160281
GTATATA42350.013.85226251
GCTCTAG39800.013.69932651
CTATACT28800.013.3842124
GGTCTAA25500.012.9914081
GTCTTAA35100.012.8792131
CGAATCG5850.012.3473859
GTACAGA59150.012.3098541
GTGTTAG31500.012.2696641
GTGCTAA33900.012.2153731
GTACTAA24300.012.2128591
GTACAGG61850.012.1072411
GATCTAG28100.011.9121621
GTATTAA35600.011.8259221
Produced by FastQC (version 0.11.5)