FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13735286
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15250.019.4232461
GGTCTAG24500.016.026251
GTGCTAG34300.015.8655881
GTTCTAG35750.015.5111161
GTCTTAG30150.015.1934891
GTATTAG24750.015.0294051
GTATATA45000.014.77195551
GTCCTAG34050.014.7682481
GTATAGG21450.014.451351
GTATAAG25850.014.2566161
GTATTAA37850.014.1045431
GTGTTAG34350.013.8371141
GGTCTAA25950.013.8034981
GTACTAA25700.013.669741
GTCCTAT26850.013.3408121
GTGCTAA30100.013.2734761
CTATACT28750.013.04284
GCCTTAG31050.012.7564391
GTACTAC20250.012.7564381
CTAAGCG8950.012.6844934
Produced by FastQC (version 0.11.5)