FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792340
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA750.001992246823.0032062
GCGTACG1052.3171644E-522.9552889
GTATACG1455.2449104E-721.4368881
GTCCTAG8800.019.2309461
TTGCGCG1603.0029873E-517.21646512
TCTATAC6550.016.319743
GTCTATA7300.016.0858231
GTACTAG4550.015.9402491
GTATAAG6850.015.6299761
TCTGTAC15900.015.3976363
GTATTAA9650.015.3896361
GTCCTAA6600.015.175441
TTAGGAC6850.015.0924524
GCTCTAG11600.014.8867271
TCTAGAC8350.014.8665043
GTATTAT8800.014.5213261
GTTCTAG11000.014.4428321
GGTCTAG7200.014.3905031
CTGTACT18700.014.0055814
GTACTAA6700.013.9179781
Produced by FastQC (version 0.11.5)