FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3762998
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3700.020.4804331
GTCCTAG8350.019.8003521
TATACTG9350.018.4199355
GTATTAG5850.018.252571
GTATAGG6250.018.1866251
GTACTAA5900.016.930281
GTGCTAG7850.015.7961411
GTCCTAA7250.015.6781241
CTAGTAC4350.015.0446643
GTTCTAG9350.014.7355561
CGTTAAA2353.2766675E-614.6571761
CTATACT7550.014.599134
TCTAGAC7800.014.5726153
GGTCTAA6400.014.5312171
GTCTTAG7450.014.3325541
TTATACT11550.014.3147314
GTGTTAG7300.014.1552171
TTATACG1951.6217252E-414.1312094
CTACGCT1951.6217252E-414.1312094
GTACTAC7100.014.0688241
Produced by FastQC (version 0.11.5)